Reagent for prothrombin time determination by clotting method (rabbit brain in a mixture with calcium chloride in a buffer with stabilizers) (Renamplastin) according to RC 21.20.23-066-05595541-2019.
The Renamplastin reagent is designed to determine the prothrombin time (PV) in venous blood plasma and calculate the prothrombin ratio (PO), prothrombin index (PI) and International Normalized Ratio (INR), as well as to determine the prothrombin by Quick in% of the norm.
ISI=1.0-1.3.
The determination of prothrombin time is a highly sensitive screening test that detects abnormalities in the external and general blood coagulation pathway (f. II, V, VII and X) and is recommended for:
- monitoring of indirect anticoagulant therapy;
- diagnostics of hereditary and acquired coagulopathies;
- diagnosis of liver diseases.
The Renamplatin is designed to work on all types of semi-automatic and automatic coagulometers and by manual method.
The principle of the method:
When added to the citrated plasma, an excess of tissue thromboplastin and calcium ions results in the formation of a fibrin clot. The time from adding the thromboplastin-calcium ion mixture to the investigated plasma to the formation of the fibrin clot depends solely on the activity of factors in the external and common pathways of coagulation: I, II, V, VII, X.
The composition of the sets:
One vial of freeze-dried Renamplastin is designed to carry out 80 determinations at a consumption of 0.1 ml of reagent solution per analysis.
One bottle of liquid Renamplastin is designed to carry out 50 determinations at a consumption of 0.1 ml of reagent solution per analysis.
Normal and pathological values of the studied hemostasis parameters should be monitored using a Prothrombin control, KM-17 or using Control plasma (pool of healthy donors) (Plasma H) , KM-1.
