Transforming reagent according to TU 9398-037-05595541-2011:
The reagent is intended for the quantitative determination of hemoglobin in blood using the hemiglobincyanide method on spectrophotometers (SF), photoelectrocolorimeters (FEC), and hemoglobinometers.
Method Principle: The method is based on the formation of a stable compound, hemiglobincyanide, from hemoglobin, the intensity of whose color is proportional to the amount of hemoglobin. The reaction occurs in two stages: under the action of potassium ferrocyanide, hemoglobin is oxidized to methemoglobin; then, under the action of sodium cyanide, methemoglobin is converted to hemiglobincyanide.
The hemiglobincyanide method is recommended by the International Committee for Standardization in Hematology. The method allows the determination of all hemoglobin derivatives except sulfhemoglobin.
Composition of the kit:
The transforming reagent is a mixture of finely ground and homogenized salts of potassium ferrocyanide, sodium cyanide, and sodium bicarbonate.
The kit is designed for conducting 2000 determinations with a consumption of 5.0 ml of the transforming reagent per analysis.
To verify the accuracy of hemoglobin determination using the hemiglobincyanide method (internal laboratory control), a set of hemoglobin control solutions (Diagem K), with the code GM-3, is required.
