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Diagem T

Reagent kit for hemoglobin determination (Diagem T) according to TU 9398-011-05595541-2009:

The Diagem T kit with a calibrator is designed for the quantitative determination of total hemoglobin in blood using the hemiglobincyanide method on spectrophotometers (SF), photoelectrocalorimeters (FEC), and hemoglobinometers.

Method Principle: The method is based on the formation of a stable compound, hemiglobincyanide, from blood hemoglobin, the intensity of the color of which is proportional to the amount of hemoglobin. The reaction occurs in two stages:

  1. Oxidation of all forms of blood hemoglobin to methemoglobin under the action of potassium ferrocyanide.
  2. Conversion of methemoglobin to hemiglobincyanide under the action of cyanide ions.

The hemiglobincyanide method is recommended by the International Committee for Standardization in Hematology. The method allows the determination of all hemoglobin derivatives except sulfhemoglobin.

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Composition of the kit:

  1. Transforming reagent (1.25 g/vial) - 5 vials,
  2. Calibrating solution of hemiglobincyanide (5 ml/ampoule) - 1 ampoule.

One kit is intended for conducting 1000 determinations with a consumption of 5.0 ml of transforming reagent per analysis.

To verify the accuracy of hemoglobin determination using the hemiglobincyanide method (internal laboratory control), a set of control solutions of hemoglobin (Diagem K), with the code GM-3, is required.