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Calibration solutions of hemiglobincyanide (Hemiglobincyanide) according to TU 9398-010-05595541-2009 are intended for calibrating instruments and determining total hemoglobin in blood using the hemiglobincyanide method with the help of a calibration curve on spectrophotometers (SF) and photoelectrocalorimeters (FEC).

Method Principle: The method is based on the formation of a stable compound, hemiglobincyanide, from blood hemoglobin, the intensity of the color of which is proportional to the amount of hemoglobin. The reaction occurs in two stages:

  1. Oxidation of all forms of blood hemoglobin to methemoglobin under the action of potassium ferrocyanide.
  2. Conversion of methemoglobin to hemiglobincyanide under the action of cyanide ions. The hemiglobincyanide method is recommended by the International Committee for Standardization in Hematology. The method allows the determination of all hemoglobin derivatives except sulfhemoglobin.
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Composition of the kit:

  1. Solutions of hemiglobincyanide (5.0 ml each) with concentrations of 200, 400, 600, and 800 mg/L, corresponding to hemoglobin concentrations in human blood of 50, 100, 150, and 200 g/L, respectively. These solutions are provided in 4 light-protective glass ampoules.

To verify the accuracy of hemoglobin determination using the hemiglobincyanide method (internal laboratory control), a set of control solutions of hemoglobin (Diagem K) produced by the "MBOOI Society of Hemophiliacs," with the code GM-3, is required.