Prothrombin calibrator

Plasma Calibrator for INR and Prothrombin Time (Prothrombin Calibrator) according to TU 9398-057-05595541-2013 is designed to determine the average normal prothrombin time (PT100%) when expressing the results of prothrombin time (PT) as the International Normalized Ratio (INR). It is also used for constructing a calibration curve correlating PT in seconds to prothrombin in percentage (PT%).

Prothrombin time determination is a highly sensitive screening test that detects abnormalities in the external pathway factors of blood coagulation (Factors II, V, VII, and X). It is recommended for:

• Monitoring therapy with oral anticoagulants.
• Diagnosing inherited and acquired coagulopathies.
• Diagnosing liver diseases.

The Prothrombin Calibrator is a pool of plasma obtained from at least 20 donors aged 20-40 years. The plasma is stabilized with HEPES-citrate buffer and subjected to lyophilization.

Method Principle: The method for determining prothrombin according to the Quik method is based on constructing a calibration curve and should be performed following the instructions provided by the reagent kit manufacturers, taking into account the requirements of the analyzer manufacturers. The calibration curve is used to determine the prothrombin value according to the Quik method (% PT) in the patient's blood plasma.

The average normal prothrombin time (PT100%), required for expressing the results of PT as INR, represents the geometric mean of the PT results in the plasma of at least 20 healthy donors. PT100% is calculated by determining the PT value in the Prothrombin Calibrator and using the PT value indicated in the passport for the Prothrombin Calibrator.