Reagent kit for determining fibrinogen content (OptiFibrinogen test) according to TU 9398-015-05595541-2008 is intended for measuring fibrinogen concentration in blood plasma using the clotting method according to Clauss on all optical-type coagulological analyzers using kaolin suspension.
Determining the fibrinogen content in human blood plasma is one of the key tests in hemostasis research. The test is designed to measure fibrinogen levels in plasma during hyperfibrinogenemia, which is associated with the severity of inflammatory, immune, and destructive processes. It is also a contributing factor to the increased risk of developing hypercoagulable syndrome, arterial thrombosis, organ infarctions, as well as in cases of acute disseminated intravascular coagulation (DIC) syndrome, during fibrinolytic therapy, and in cases of inherited hypo- and dysfibrinogenemias.
Method principle: The clotting time of diluted 10-fold citrated blood plasma is measured upon the addition of an excess of thrombin. In this system, the fibrinogen concentration in the plasma solely determines the clotting time, which is determined using a calibration curve of dilutions of plasma-calibrator with a known fibrinogen content.
The thrombin contains a heparin inhibitor, allowing the use of this reagent for measuring fibrinogen content in heparinized plasma samples from patients.
Composition of the kit:
One kit is intended for 320 analyses with a consumption of 0.05 ml of thrombin per analysis.
Normal and pathological values of fibrinogen should be monitored using Control Plasma (pool of healthy donors) (Plasma H), code KM-1.
