Coagulo-Express

Reagent kit for determining Activated Partial Thromboplastin Time (APTT) (Coagulo-Express) according to TU 9398-014-05595541-2007. Designed for determining APTT in blood plasma on semi-automatic and automatic coagulometers capable of detecting clot formation in the presence of kaolin.

Activated Partial Thromboplastin Time (APTT) is one of the most common and sensitive tests for detecting a wide range of coagulation disorders. APTT varies depending on various quantities of fibrinogen, prothrombin activity, factors V, VIII, IX, X, XI, XII, other contact factors, and the presence of specific inhibitors of coagulation factors VIII and IX. APTT is a widely used method for monitoring the effectiveness of parenteral administration of heparin, where clotting time increases proportionally to the level of heparin. In patients taking oral anticoagulants, levels of circulating factors II, VII, IX, and X are reduced, leading to an increase in APTT. In the presence of nonspecific inhibitors, such as lupus anticoagulant, APTT may be prolonged.

Method Principle: Incubating plasma with an optimally standardized ratio of phospholipids and kaolin activates the factors of the internal coagulation pathway. Adding calcium ions to the system neutralizes the citrate in the plasma and allows for the formation of a fibrin clot. During APTT measurement, the time from the addition of calcium ions to the formation of a clot is recorded.