APTT-silicum reagent

Reagent for Activated Partial Thromboplastin Time (APTT) Determination using the Clotting Method (APTT-Silicon Reagent) according to TU 21.20.23-063-05595541-2017. The kit is designed for the determination of activated partial thromboplastin time (hereinafter referred to as 'APTT') in blood plasma to detect a wide range of coagulation disorders and monitor heparin therapy. Suitable for use on all types of semi-automatic and automatic coagulometers.

APTT determination is one of the most common and sensitive screening tests for detecting a wide range of coagulation disorders. Shortening of APTT may indicate thrombophilia, thromboembolism, and thromboses. Prolongation of APTT is associated with inherited or acquired deficiencies of fibrinogen, prothrombin, factors V, VIII, IX, X, XI, XII, contact factors, the presence of specific coagulation inhibitors, oral anticoagulant use, and treatment with heparin and hirudin, as well as the presence of lupus anticoagulant and DIC syndrome.

The APTT-silicon reagent is sensitive to the presence of heparin with minimal activity, equal to 0.1 IU/ml, allowing the use of this reagent for monitoring heparin therapy.

Principle of the method: The method is based on determining the clotting time of the investigated blood plasma in the presence of an activator of the internal coagulation pathway and phospholipids. Incubation of plasma with a surface activator leads to the activation of contact factors and sequential activation of factors in the internal and common pathways of blood coagulation on the surface of phospholipid vesicles. During the APTT measurement, the time from the addition of calcium ions to the formation of a clot is recorded