Reagent for Prothrombin Time Determination (Thromboplastin) according to TU 9398-038-05595541-2011
The Thromboplastin reagent is intended for determining the prothrombin time (PT) in venous blood plasma and calculating the prothrombin ratio (PR), prothrombin index (PI), and International Normalized Ratio (INR), as well as determining prothrombin according to Quick in % of the norm.
The determination of prothrombin time is a highly sensitive and simple screening test that detects abnormalities in the external and common pathways of blood coagulation (Factors II, V, VII, and X). It is recommended for:
Monitoring therapy with indirect anticoagulants;
Diagnosing hereditary and acquired coagulopathies;
Diagnosing liver diseases.
The Thromboplastin kit is designed to work on all types of semi-automatic and automatic coagulometers and can be used with manual methods.
Method principle:
When added to citrated plasma, an excess of tissue thromboplastin and calcium ions results in the formation of a fibrin clot. The time from adding the thromboplastin-calcium ion mixture to the investigated plasma to the formation of the fibrin clot depends solely on the activity of factors in the external and common pathways of coagulation: I, II, V, VII, X.
Kit Composition: Thromboplastin – lyophilized water-soluble reagent obtained from rabbit brain. • Thromboplastin (2.5 ml/vial) – 10 vials. One Thromboplastin kit is intended for conducting 500 analyses with a consumption of 0.1 ml of thromboplastin per analysis. Required additional reagent not included in the kit: 0.025 M calcium chloride solution (5 or 10 ml), code P-9, P-9/1. Normal and pathological values of prothrombin according to Quick and INR should be monitored using the Prothrombin Control kit, code KM-17, or Control Plasma (pool of healthy donors) (Plasma N), code KM-1.
